Composite
CAHS 1

Part:BBa_K2623014:Design

Designed by: Hongling Liu   Group: iGEM18_XMU-China   (2018-09-28)


Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 375
    Illegal NheI site found at 1679
    Illegal NheI site found at 1702
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2880
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1376
    Illegal AgeI site found at 1488
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used Gibson assembly to construct the circuit.


Source

This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini)

References